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Structured Review

Addgene inc plix402
Plix402, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plix-402/pLIX_402+(Plasmid+%2341394)/bio_rxiv__64898__2026__03__06__710102-118-41-42
Average 95 stars, based on 77 article reviews
plix402 - by Bioz Stars, 2026-10
95/100 stars

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Related Articles

Construct:

Article Title: Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation
Article Snippet: Constructs for Tet-inducible expression of 1G4 ab, 2H5 ab, zWTandmutants, affinity enhanced 1G4 (wtc51 andQM-a), zTST and constitutive 1G4 ab and 868 TCR To generate an inducible expression system for 1G4 abWT and 1G4 aWT/bA291, the sequence coding for the self- cleavable single polypeptide b-HA-F2A-a-FLAG was cloned into the doxycycline inducible Gateway cloning plasmid pLIX-402 (Addgene) following the manufacturer’s guidelines for Gateway cloning.

Expressing:

Article Title: Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation
Article Snippet: Constructs for Tet-inducible expression of 1G4 ab, 2H5 ab, zWTandmutants, affinity enhanced 1G4 (wtc51 andQM-a), zTST and constitutive 1G4 ab and 868 TCR To generate an inducible expression system for 1G4 abWT and 1G4 aWT/bA291, the sequence coding for the self- cleavable single polypeptide b-HA-F2A-a-FLAG was cloned into the doxycycline inducible Gateway cloning plasmid pLIX-402 (Addgene) following the manufacturer’s guidelines for Gateway cloning.

Sequencing:

Article Title: Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation
Article Snippet: Constructs for Tet-inducible expression of 1G4 ab, 2H5 ab, zWTandmutants, affinity enhanced 1G4 (wtc51 andQM-a), zTST and constitutive 1G4 ab and 868 TCR To generate an inducible expression system for 1G4 abWT and 1G4 aWT/bA291, the sequence coding for the self- cleavable single polypeptide b-HA-F2A-a-FLAG was cloned into the doxycycline inducible Gateway cloning plasmid pLIX-402 (Addgene) following the manufacturer’s guidelines for Gateway cloning.

Clone Assay:

Article Title: Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation
Article Snippet: Constructs for Tet-inducible expression of 1G4 ab, 2H5 ab, zWTandmutants, affinity enhanced 1G4 (wtc51 andQM-a), zTST and constitutive 1G4 ab and 868 TCR To generate an inducible expression system for 1G4 abWT and 1G4 aWT/bA291, the sequence coding for the self- cleavable single polypeptide b-HA-F2A-a-FLAG was cloned into the doxycycline inducible Gateway cloning plasmid pLIX-402 (Addgene) following the manufacturer’s guidelines for Gateway cloning.

Cloning:

Article Title: Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation
Article Snippet: Constructs for Tet-inducible expression of 1G4 ab, 2H5 ab, zWTandmutants, affinity enhanced 1G4 (wtc51 andQM-a), zTST and constitutive 1G4 ab and 868 TCR To generate an inducible expression system for 1G4 abWT and 1G4 aWT/bA291, the sequence coding for the self- cleavable single polypeptide b-HA-F2A-a-FLAG was cloned into the doxycycline inducible Gateway cloning plasmid pLIX-402 (Addgene) following the manufacturer’s guidelines for Gateway cloning.

Plasmid Preparation:

Article Title: Allosteric activation of T cell antigen receptor signaling by quaternary structure relaxation
Article Snippet: Constructs for Tet-inducible expression of 1G4 ab, 2H5 ab, zWTandmutants, affinity enhanced 1G4 (wtc51 andQM-a), zTST and constitutive 1G4 ab and 868 TCR To generate an inducible expression system for 1G4 abWT and 1G4 aWT/bA291, the sequence coding for the self- cleavable single polypeptide b-HA-F2A-a-FLAG was cloned into the doxycycline inducible Gateway cloning plasmid pLIX-402 (Addgene) following the manufacturer’s guidelines for Gateway cloning.



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